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Rabbit Anti-TNF alpha antibody (bs-10802R)
~~~促销,代码SSY221101~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1138.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-10802R
英文名称 TNF alpha
中文名称 肿瘤坏死因子-α/TNFα/TNF-α抗体
别    名 Tumor necrosis factor-α; TNFalpha; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a; TNF-alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor Alpha; TNFA_HUMAN.  
Specific References  (23)     |     bs-10802R has been referenced in 23 publications.
[IF=5.893]   WB, IHC ;  mouse, human.  
[IF=14.593] Bin Wang. et al. Targeted delivery of a STING agonist to brain tumors using bioengineered protein nanoparticles for enhanced immunotherapy. Bioact Mater. 2022 Mar;:  IF ;  Mouse.  
[IF=7.813] Shengyan Yang. et al. Extracellular vesicles delivering nuclear factor I/C for hard tissue engineering: Treatment of apical periodontitis and dentin regeneration:. J Tissue Eng. 2022;():  IHC ;  Rat.  
[IF=6.529] Mennatallah A. Gowayed. et al. The α7 nAChR allosteric modulator PNU-120596 amends neuroinflammatory and motor consequences of parkinsonism in rats: Role of JAK2/NF-κB/GSk3β/ TNF-α pathway. Biomed Pharmacother. 2022 Apr;148:112776  IHC ;  Rat.  
[IF=5.59] Li, Jiayi. et al. Activation of the GPX4/TLR4 Signaling Pathway Participates in the Alleviation of Selenium Yeast on Deltamethrin-Provoked Cerebrum Injury in Quails. Mol Neurobiol. 2022 Mar;:1-16  WB ;  Quails.  
[IF=4.36] Hong Zhao. et al. Protective effect of flavonoids extract of Hippophae rhamnoides L. on alcoholic fatty liver disease through regulating intestinal flora and inhibiting TAK1/p38MAPK/p65NF-κB pathway. J Ethnopharmacol. 2022 Jun;292:115225  WB ;  Mouse.  
[IF=2.408] Jingru Qin. et al. In-silico analysis identifies the anti-liver injury targets of diammonium glycyrrhizinate: validated in perfluorooctanoic acid-lesioned mouse model. 2022 Mar 31  IHC ;  Mouse.  
[IF=2.248] Wang Yulong. et al. LncRNA GAS5 promotes abdominal aortic aneurysm formation through regulating the miR-185-5p/ADCY7 axis. Anti-Cancer Drug. 2022 Mar;33(3):225-234  WB ;  Human.  
[IF=3.905] Fen Xu. et al. Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway. Exp Cell Res. 2022 Mar;412:113025  IF ;  Rat.  
[IF=9.229] Jiarong Yang. et al. Mitigation Effects of Selenium Nanoparticles on Depression-Like Behavior Induced by Fluoride in Mice via the JAK2-STAT3 Pathway. Acs Appl Mater Inter. 2022;14(3):3685–3700  WB ;  Mouse.  
[IF=7.658] Yongbo Kang. et al. Lactobacillus acidophilus ameliorates obesity in mice through modulation of gut microbiota dysbiosis and intestinal permeability. Pharmacol Res. 2021 Dec;:106020  IHC ;  Mouse.  
[IF=3.24] Chang Chen. et al. H2O2 gel bleaching induces cytotoxicity and pain conduction in dental pulp stem cells via intracellular reactive oxygen species on enamel/dentin disc. Plos One. 2021 Sep;16(9):e0257221  IF ;  rat.  
[IF=6.291] Si-Cheng Zhao. et al. Nickel sulfate exposure induces ovarian inflammation and fibrosis and decreases oocyte quality in mice. Ecotox Environ Safe. 2021 Nov;224:112634  IHC ;  Mouse.  
[IF=1.813] Jiang Yan. et al. Exploring the Mechanism of Berberine Intervention in Ulcerative Colitis from the Perspective of Inflammation and Immunity Based on Systemic Pharmacology. Evid-Based Compl Alt. 2021;2021:9970240  IHC ;  Rat.  
[IF=3.216] Ji-sheng Wang. et al. Study on the Mechanism of Jiawei Shengjiang Powder in Improving Male Asthma-Induced Asthenospermia Based on Network Pharmacology and Bioinformatics. Drug Des Dev Ther. 2021 Mar;15:1245-1259  WB ;  Rat.  
[IF=5.893] Ju-Tao Yu. et al. DNA methylation of FTO promotes renal inflammation by enhancing m6A of PPAR-α in alcohol-induced kidney injury. Pharmacol Res. 2021 Jan;163:105286  IHC ;  Mouse.  
[IF=1.918] Wang J et al. Effect of Asthma on Erectile Dysfunction in Rats as Determined by Biological Network AnalysisMed Sci Monit.2020 Dec 20;26:e927491.  WB ;  Rat.  
[IF=1.574] Song L et al. Schisandrin ameliorates cognitive deficits, endoplasmic reticulum stress and neuroinflammation in STZ-induced Alzheimer's disease rats. Exp Anim . 2020 Aug 5;69(3):363-373.  IHC ;  rat.  
[IF=2.998] Lee M et al. Acute changes in the retina and central retinal artery with methamphetamine. Exp Eye Res. 2020 Feb 7;193:107964.  WB&IHF-P ;  Mouse.  
[IF=0] Artono Surarto B et al. The Association of IL-1 Alpha Level and TNF Alpha Expressions on Bone Destruction in Chronic Suppurative Otitis Media and Cholesteatoma. Indian Journal of Otolaryngology and Head & Neck Surgery.2019.  IHC ;  Human.  
[IF=3.265] Meng HW et al. 4-Methylcoumarin-[5,6-g]-hesperetin attenuates inflammatory responses in alcoholic hepatitis through PPAR-γ activation. Toxicology. 2019 Apr 3;421:9-21.  WB ;  Mouse.  
[IF=7.233] Chen X et al. A photothermal-triggered nitric oxide nanogenerator combined with siRNA for precise therapy of osteoarthritis by suppressing macrophage inflammation.Nanoscale. 2019 Apr 4;11(14):6693-6709.  IHC-P&IF ;  Mouse.  
[IF=3.83] Cheng et al. miR-203 Inhibits Alcohol-Induced Hepatic Steatosis by Targeting Lipin1. (2018) Front.Pharmacol. 9:275  WB ;  Mouse.  
研究领域 肿瘤  心血管  免疫学  信号转导  细胞凋亡  生长因子和激素  细胞因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse,  (predicted: Rat, )
产品应用 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 17/26kDa
细胞定位 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 Recombinant human TNF alpha protein: 77-233/233 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020]

Function:
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Subunit:
Homotrimer. Interacts with SPPL2B.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein.
Tumor necrosis factor, membrane form: Membrane; Single-pass type II membrane protein.
Tumor necrosis factor, soluble form: Secreted.
C-domain 1: Secreted.
C-domain 2: Secreted.

Post-translational modifications:
The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

DISEASE:
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

Similarity:
Belongs to the tumor necrosis factor family.

SWISS:
P01375

Gene ID:
7124

Database links:

Entrez Gene: 7124 Human

Entrez Gene: 21926 Mouse

Omim: 191160 Human

SwissProt: P01375 Human

SwissProt: P06804 Mouse

Unigene: 241570 Human

Unigene: 1293 Mouse



产品图片
Blank control:Raw264.7.
Primary Antibody (green line): Rabbit Anti-TNF alpha antibody (bs-10802R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were treated with LPS (1 ug/mL, 18 hr/6 hr) and Brefeldin A (300 ng/mL, last 3 hr of stimulation).The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:THP-1.
Primary Antibody (green line): Rabbit Anti-TNF alpha antibody (bs-10802R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were treated with TPA (80 nM, overnight) and then treated with LPS (1 ug/mL, 18 hr/6 hr) and Brefeldin A (300 ng/mL, last 3 hr of stimulation) .The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-TNF alpha antibody (bs-10802R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature.Acquisition of 20,000 events was performed.
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