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RabbitAnti-Calreticulin antibody (bs-5913R)
订购QQ:  400-901-9800
说明书: 50ul  100ul  200ul

产品编号 bs-5913R
英文名称 Calreticulin
中文名称 钙网蛋白抗体
别    名 Autoantigen RO; CALR; CALR protein; CALR_HUMAN; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; SSA.   
Specific References  (14)     |     bs-5913R has been referenced in 14 publications.
111 [IF=1.58] Chen, Beilei, et al. "Calreticulin Binds to Fas Ligand and Inhibits Neuronal Cell Apoptosis Induced by Ischemia-reperfusion Injury." BioMed Research International.  Human.  222
111 [IF=5.008] Tanaka, Mamoru, et al. "Immunogenic cell death due to a new photodynamic therapy (PDT) with glycoconjugated chlorin (G-chlorin)." Oncotarget (2016).  IHC-P ;  Mouse.  222
111 [IF=1.51] Erbas et al. Oxytocin alleviates cisplatin-induced renal damage in rats. (2014) Iran.J.Basic.Med.Sci. 17:747-52  IHC ;  Rat.  222
111 [IF=14.588] An J et al. Nanoenabled Disruption of Multiple Barriers in Antigen Cross-Presentation of Dendritic Cells via Calcium Interference for Enhanced Chemo-Immunotherapy. ACS Nano 2020 06 23;146(6)  Other ;  
111 [IF=5.88] Rui X et al. Imperative and effective reversion of synovial hyperplasia and cartilage destruction in rheumatoid arthritis through multiple synergistic effects of O2 and Ca2+. Mater Sci Eng C Mater Biol Appl.2020 Sep;114:111058.  IF ;  Rat.  222
111 [IF=4.556] Hiroshi Fukushima. et al. Combination of Cisplatin and Irradiation Induces Immunogenic Cell Death and Potentiates Postirradiation Anti–PD-1 Treatment Efficacy in Urothelial Carcinoma. Int J Mol Sci. 2021 Jan;22(2):535  IHC ;  Mouse.  222
111 [IF=13.281] Kai Sun. et al. Reinforcing the Induction of Immunogenic Cell Death Via Artificial Engineered Cascade Bioreactor-Enhanced Chemo-Immunotherapy for Optimizing Cancer Immunotherapy. 2021 Aug 06  FC ;  Mouse.  222
111 [IF=15.881] Yuting Shen. et al. Tailoring Chemoimmunostimulant Bioscaffolds for Inhibiting Tumor Growth and Metastasis after Incomplete Microwave Ablation. Acs Nano. 2021;XXXX(XXX):XXX-XXX  IF ;  Mouse.  222
111 [IF=12.479] Pan Zhang. et al. The programmed site-specific delivery of LY3200882 and PD-L1 siRNA boosts immunotherapy for triple-negative breast cancer by remodeling tumor microenvironment. BIOMATERIALS. Biomaterials. 2022 Apr;:121518  IF,FC ;  Mouse.  222
111 [IF=10.633] Kai Sun. et al. Salicylic acid-based hypoxia-responsive chemodynamic nanomedicines boost antitumor immunotherapy by modulating immunosuppressive tumor microenvironment. ACTA BIOMATER. 2022 Jun;:  IF ;  Mouse.  222
111 [IF=11.467] Yanhua Zhai. et al. Self-activated arsenic manganite nanohybrids for visible and synergistic thermo/immuno-arsenotherapy. J CONTROL RELEASE. 2022 Oct;350:761  IF, FC ;  Mouse.  222
111 [IF=18.027] Nguyen Thi Nguyen. et al. Amplified Fenton-Based Oxidative Stress Utilizing Ultraviolet Upconversion Luminescence-Fueled Nanoreactors for Apoptosis-Strengthened Ferroptosis Anticancer Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXX  IF ;  Mouse.  222
111 [IF=6.656] Ning Han. et al. Dihydroartemisinin elicits immunogenic death through ferroptosis-triggered ER stress and DNA damage for lung cancer immunotherapy. PHYTOMEDICINE. 2023 Jan;:154682  FCM,IF ;  Mouse.  222
111 [IF=5.714] Ting-Ting Yu. et al. Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage. INT IMMUNOPHARMACOL. 2023 Feb;115:109661  FCM,IF ;  Mouse,Human.  222
研究领域 信号转导  细胞类型标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 44kDa
细胞定位 细胞浆 细胞膜 细胞外基质 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Calreticulin: 101-200/417 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020]

Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 (By similarity). Interacts with NR3C1 and TRIM21. Interacts with GABARAP.

Subcellular Location:
Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface.

Belongs to the calreticulin family.


Gene ID:

Database links:

Entrez Gene: 41166 Fruit fly (Drosophila melanogaster)

Entrez Gene: 811 Human

Entrez Gene: 12317 Mouse

Entrez Gene: 100009050 Rabbit

Entrez Gene: 64202 Rat

Omim: 109091 Human

SwissProt: P29413 Fruit fly (Drosophila melanogaster)

SwissProt: P27797 Human

SwissProt: P14211 Mouse

SwissProt: P15253 Rabbit

SwissProt: P18418 Rat

Unigene: 2457 Fruit fly (Drosophila melanogaster)

Unigene: 515162 Human

Unigene: 1971 Mouse

Unigene: 467043 Mouse

Unigene: 974 Rat

Liver (Mouse) Lysate at 40 ug
Primary: Anti-Calreticulin   (bs-5913R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 49 kD
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse NIH/3T3 cell lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Human SH-SY5Y cell lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human K562 cell lysates
Lane 7: Human HL-60 cell lysates
Lane 8: Human A431 cell lysates
Primary: Anti-Calreticulin (bs-5913R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 48 kDa
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Calreticulin Polyclonal Antibody, FITC conjugated (bs-5913R-FITC) 1:100, 40 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Cell: Hela
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: bs-5913R) on Hela(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-Calreticulin antibody (bs-5913R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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