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Rabbit Anti-VEGFA antibody (bs-1313R)
~~~促销,代码SSY221101~~~
订购热线:400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-1313R
英文名称 VEGFA
中文名称 血管内皮生长因子抗体
别    名 Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; VEGF-A; Vascular permeability factor (VPF);   
Specific References  (42)     |     bs-1313R has been referenced in 42 publications.
[IF=6.832] Liu, Jingyu. et al. Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladder. Stem Cell Res Ther. 2022 Dec;13(1):1-16  WB ;  Rat.  
[IF=3.528] Jie Wang. et al. A radiomics model based on DCE-MRI and DWI may improve the prediction of estimating IDH1 mutation and angiogenesis in gliomas. Eur J Radiol. 2022 Feb;147:110141  IHC ;  Human.  
[IF=9.933] Qiuyue Ding. et al. Bioinspired Multifunctional Black Phosphorus Hydrogel with Antibacterial and Antioxidant Properties: A Stepwise Countermeasure for Diabetic Skin Wound Healing. 2022 Feb 19  WB ;  Human.  
[IF=9.078] Lin Zou. et al. Icariin-releasing 3D printed scaffold for bone regeneration. Compos Part B-Eng. 2022 Mar;232:109625  IHC ;  Rat.  
[IF=3.411] Li Zhi-xin. et al. circGLI3 Inhibits Oxidative Stress by Regulating the miR-339-5p/VEGFA Axis in IPEC-J2 Cells. Biomed Res Int. 2021;2021:1086206  WB ;  Pig.  
[IF=4.268] Jian-xia Wen. et al. Therapeutic Effects and Potential Mechanism of Dehydroevodiamine on N-Methyl-N′-Nitro-N-Nitrosoguanidine-Induced Chronic Atrophic Gastritis. Phytomedicine. 2021 Jun;:153619  WB,IHC ;  Rat.  
[IF=7.242] Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 Mar;:  WB,IF,IHC ;  Mouse.  
[IF=3.216] Yanhong Wang. et al. Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats. Drug Des Dev Ther. 2020; 14: 4825–4834  IHC ;  Rat.  
[IF=2.283] Ismet Hortu. et al. Oxytocin and cabergoline alleviate ovarian hyperstimulation syndrome (OHSS) by suppressing vascular endothelial growth factor (VEGF) in an experimental model. 2020 Nov 02  IHC ;  Rat.  
[IF=4.848] Hu Y et al. Non-Invasive Estimation of Glioma IDH1 Mutation and VEGF Expression by Histogram Analysis of Dynamic Contrast-Enhanced MRIFront Oncol.2020 Dec 8;10:593102.  IHC ;  Human.  
[IF=4.784] Ding J et al. Anti-angiogenic effect of a chemically sulfated polysaccharide from Phellinus ribis by inhibiting VEGF/VEGFR pathway. Int J Biol Macromol. 2020 Mar 11;154:72-81.  IHC,WB ;  mouse.  
[IF=2.705] Zhang Q et al. The circular RNA hsa_circ_0007623 acts as a sponge of microRNA-297 and promotes cardiac repair. Biochem Biophys Res Commun. 2020 Mar 19;523(4):993-1000.  IHC-P ;  Mouse.  
[IF=2.479] Wang H et al. The effects of a hirudin/liposome complex on a diabetic nephropathy rat model. BMC Complement Altern Med. 2019 Jun 6;19(1):118.  IHC-P ;  Rat.  
[IF=1.694] Yahuafai J et al. Anticancer Efficacy of the Combination of Berberine and PEGylated Liposomal Doxorubicin in Meth A Sarcoma-Bearing Mice.Biol Pharm Bull. 2018;41(7):1103-1106.  WB ;  Human.  
[IF=3.349] Bi SX et al. The antitumour growth and antiangiogenesis effects of xanthatin in murine glioma dynamically evaluated by dynamic contrast‐enhanced magnetic resonance imaging.(2018) Phytother Res. Oct 22.  IHC ;  Rat、Mouse.  
[IF=2.159] Liu X et al. The impact of ALDH2 activation by Alda-1 on the expression of VEGF in the hippocampus of a rat model of post-MI depression. Neurosci Lett. 2018 May 1;674:156-161.  WB&IHC ;  Rat.  
[IF=0] Ren et al. Interferon-γ and celecoxib inhibit lung-tumor growth through modulating M2/M1 macrophage ratio in the tumor microenvironment. (2014) Drug.Des.Devel.Ther. 8:1527-38  IHC ;  Mouse.  
[IF=1.075] Yao, Qian, Yingxia Zhou, and Bing Li. "Thymoquinone (TQ) inhibits corneal neovascularization in a rabbit model." Int J Clin Exp Med 9.8 (2016): 15907-15913.  IHC-P ;  Rabbit.  
[IF=1.922] Chi et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. (2018) Mol.Med.Rep. 17:6337-6344  IHC ;  Human.  
[IF=2.1] Zhenjie Huang. et al. Protective effects of different anti‑inflammatory drugs on tracheal stenosis following injury and potential mechanisms. Mol Med Rep. 2021 May;23(5):1-11  WB ;  Rabbit.  
[IF=6.291] Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-κB pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166  ELISA ;  Bovine.  
[IF=5.923] Liang Hu. et al. Transient Activation of Hedgehog Signaling Inhibits Cellular Senescence and Inflammation in Radiated Swine Salivary Glands through Preserving Resident Macrophages. Int J Mol Sci. 2021 Jan;22(24):13493  WB,IHC ;  Pig.  
[IF=4.064] Ming Ni. et al. Sox11 Modified Tendon-Derived Stem Cells Promote the Repair of Osteonecrosis of Femoral Head:. Cell Transplant. 2021;():  WB ;  Rabbit.  
[IF=6.684] Huihua Kai. et al. LncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing VEGF Gene Transcription via Enhancing H3K9 Deacetylation by Recruiting HDAC6. Front Cell Dev Biol. 2021; 9: 701628  WB ;  Human.  
[IF=3.375] Shun Zhang. et al. PPARγ induces the paroxysm of endometriosis by regulating the transcription of MAT2A gene. Am J Transl Res. 2021; 13(3): 1377–1388  IHC ;  Human.  
[IF=2.73] Ling Zhou. et al. Psoriatic mesenchymal stem cells stimulate the angiogenesis of human umbilical vein endothelial cells in vitro. Microvasc Res. 2021 Jul;136:104151  WB ;  Human.  
[IF=5.201] Qiong Li. et al. CD73+ Mesenchymal Stem Cells Ameliorate Myocardial Infarction by Promoting Angiogenesis. Front Cell Dev Biol. 2021; 9: 637239  WB,IHC ;  Rat.  
[IF=10.652] Pei Liu. et al. Biphasic CK2.1-coated β-glycerophosphate chitosan/LL37-modified layered double hydroxide chitosan composite scaffolds enhance coordinated hyaline cartilage and subchondral bone regeneration. Chem Eng J. 2021 Aug;418:129531  IHC ;  Rabbit.  
[IF=4.197] Xuewen Qian. et al. Acidosis induces synovial fibroblasts to release vascular endothelial growth factor via acid-sensitive ion channel 1a. Lab Invest. 2020 Aug;101(3):280-291  WB,IHC ;  Human.  
[IF=3.375] Wei P. et al. Nintedanib ameliorates tracheal stenosis by activating HDAC2 and suppressing IL-8 and VEGF in rabbit.. Am J Transl Res. 2020 Aug;12(8):4739-4748  WB ;  Rabbit.  
[IF=2.055] Li-Hua Muet al. Antiangiogenic effects of AG36, a triterpenoid saponin from Ardisia gigantifolia stapf. J Nat Med . 2020 Sep;74(4):732-740.  WB ;  Human.  
[IF=1.918] Mao XB et al. Sirtuin (Sirt) 3 Overexpression Prevents Retinopathy in Streptozotocin-Induced Diabetic Rats. Med Sci Monit . 2020 May 27;26:e920883.  WB ;  rat.  
[IF=1.2] Özel F et al. Protective effect of alpha lipoic acid on 4-vinylcyclohexene diepoxide induced primary ovarian failure in female rats. Taiwan J Obstet Gynecol. 2020 Mar;59(2):293-300.  IHC ;  rat.  
[IF=3.973] Liu N et al. Polyphotosensitizer Nanogels for GSH-Responsive Histone Deacetylase Inhibitors delivery and enhanced Cancer Photodynamic Therapy. Colloids Surf B Biointerfaces. 2019 Dec 23;188:110753.  IHC-P ;  Mouse.  
[IF=3.44] Han Y et al. Studies on bacterial cellulose/poly vinyl alcohol (BC/PVA) hydrogel composites as tissue-engineered corneal stroma. Biomed Mater. 2019 Nov 12.  IHC-P ;  Rabbit.  
[IF=2.807] Ma WJ et al. Biliary antibiotics irrigation for E. coli-induced chronic proliferative cholangitis and hepatolithiasis: A pathophysiological study in rabbits. Clin Res Hepatol Gastroenterol. 2019 Aug 13. pii: S2210-7401(19)30173-1.  WB ;  Rabbit.  
[IF=3.103] Li R et al. Lithium chloride promoted hematoma resolution after intracerebral hemorrhage through GSK-3β-mediated pathways-dependent microglia phagocytosis and M2-phenotype differentiation, angiogenesis and neurogenesis in a rat model. Brain Res Bull. 2019 Jul 17;152:117-127.  WB ;  Rat.  
[IF=1.872] Li Z et al. Angiogenesis and bone regeneration by allogeneic mesenchymal stem cell intravenous transplantation in rabbit model of avascular necrotic femoral head. J Surg Res. 2013 Jul;183(1):193-203.  IHC-P ;  Rabbit.  
[IF=3.115] Zhen-zhenChen et al. Bu Yang Huan Wu Decoction prevents reperfusion injury following ischemic stroke in rats via inhibition of HIF-1 α, VEGF and promotion β-ENaC expression (2018)Journal of Ethnopharmacology Sep 13;228:70-81.  WB ;  Rat.  
[IF=1.766] Yuan X et al. Significance of nuclear magnetic resonance combined with Ki-67 and VEGF detection in the diagnosis and prognosis evaluation of brain glioma.J BUON. 2018 Mar-Apr;23(2):410-415.  IHC-P ;  Human.  
[IF=0] Gautam et al. Implantation of autologous adipose-derived cells reconstructs functional urethral sphincters in rabbit cryoinjured urethra. (2014) Tissue.Eng.Part.. 20:1971-9  IHC-P ;  Rabbit.  
[IF=1.69] Li et al. Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation. (2017) Mol.Med.Rep. 16:4455-4462  IHC-P, WB ;  Human, Rat.  
研究领域 肿瘤  信号转导  生长因子和激素  血管内皮细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Rabbit,  (predicted: Chicken, Dog, Pig, Cow, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 23kDa
细胞定位 细胞外基质 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human VEGF: 102-213/213 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.

Function:
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Subunit:
Homodimer; disulfide-linked. Also found as heterodimer with PGF.

Subcellular Location:
Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.

Tissue Specificity:
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.

DISEASE:
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

Similarity:
Belongs to the PDGF/VEGF growth factor family.

SWISS:
P15692

Gene ID:
7422

Database links:

Entrez Gene: 403802 Dog

Entrez Gene: 7422 Human

Entrez Gene: 22339 Mouse

Entrez Gene: 83785 Rat

Omim: 192240 Human

SwissProt: Q9MYV3 Dog

SwissProt: P15692 Human

SwissProt: Q00731 Mouse

SwissProt: P16612 Rat

Unigene: 73793 Human

Unigene: 282184 Mouse

Unigene: 1923 Rat




血管内皮细胞生长因子(VEGF)是一种特异作用于血管内皮细胞的多功能细胞因子,它能引起血管通透性增加,引起细胞外基质成分改变,诱导血管形成.在炎症、创伤愈合、心脏缺血、动脉粥样硬化、糖尿病性视网膜病变及肿瘤形成等与血管生成和病变有关的诸多病理过程中起重要作用.
VEGF与血管生成有关,因而与肿瘤生长有很大关系,近年来受到很多关注。
产品图片
Sample:Kidney(Mouse) Lysate at 30 ug
Primary: Anti-VEGF (bs-1313R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 27 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-VEGF Polyclonal Antibody, Unconjugated(bs-1313R) 1:300, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rabbit liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-VEGF Polyclonal Antibody, Unconjugated(bs-1313R) 1:400, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-VEGF Polyclonal Antibody, Unconjugated(bs-1313R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rabbit meniscus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-VEGF Polyclonal Antibody, Unconjugated(bs-1313R) 1:300, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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