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RabbitAnti-GFP antibody (bs-0890R)
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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-0890R
英文名称 GFP
中文名称 绿色荧光蛋白抗体
别    名 Green fluorescence protein.  绿色荧光蛋白 GFP; 绿色荧光蛋白 (GFP);
Specific References  (2)     |     bs-0890R has been referenced in 2 publications.
[IF=4.545] Liu Y et al. The synergistic effect of NELL1 and adipose-derived stem cells on promoting bone formation in osteogenesis imperfecta treatment. Biomed Pharmacother. 2020 Aug;128:110235.  IF ;  Mouse.  
[IF=1.665] Karim Rahimi. et al. Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines. In Vitro Cell Dev-An. 2020 Dec;56(10):896-905  IHC ;  Mouse.  
产品类型 标签抗体 
研究领域 细胞生物  免疫学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Recombinant protein
产品应用 WB=1:1000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from belt jellyfish Green Fluorecent protein : 25-120/238 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Function:
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Subunit:
Monomer.

Tissue Specificity:
Photocytes.

Post-translational modifications:
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

Similarity:
Belongs to the GFP family.

SWISS:
P42212

Gene ID:
107331116

Database links:




GFP在科学研究上有着惊人的用途,因为它能够使我们直接看到细胞内部的运动,情况。在任何指定的时间我们都可以轻易地找出GFP在哪儿:你只需要用紫外光去照射,这时所有的GFP都将发出鲜艳的绿色。
不妨做个实验:你把GFP连接到你有兴趣观察的任何对象上。比如,你可以把它连接到一种病毒上。然后,随着病毒在宿主体内不断扩散,你就可以通过跟踪发出的绿光来观察病毒的扩散途径;或者你把它接合到一种蛋白质上并通过显微镜观察它在细胞内部的移动。
产品图片
Sample:
Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
Lane 2: Control (-) E.coli Lysate at 4 ug
Primary: Anti-GFP (bs-0890R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
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