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Rabbit Anti-Osteocalcin antibody (bs-0470R)
~~~促销,代码SSY221101~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-0470R
英文名称 Osteocalcin
中文名称 骨钙蛋白/骨钙素抗体
别    名 Osteocalcin Propeptide; Gamma-carboxyglutamic acid-containing protein; Bone Gla-protein; BGLAP; Bone gamma carboxyglutamate protein; BGLAP; BGP; Bone gamma carboxyglutamate gla protein osteocalcin; Bone gamma carboxyglutamate protein; Bone Gla protein; Gamma carboxyglutamic acid containing protein; OC; PMF1; OSTCN_HUMAN.  
Specific References  (22)     |     bs-0470R has been referenced in 22 publications.
[IF=3.68] Zhang, Ping, et al.  WB ;  Rat.  
[IF=5.344] Ling-Kun Zhang. et al. Growth factors regional patterned and photoimmobilized scaffold applied to bone tissue regeneration. J Mater Chem B. 2020 Dec;8(48):10990-11000  IHC ;  Rat.  
[IF=7.328] Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359  IHC ;  Rat.  
[IF=2.633] Jingzhi Li. et al. TGF-β2 and TGF-β1 differentially regulate the odontogenic and osteogenic differentiation of mesenchymal stem cells. Arch Oral Biol. 2022 Mar;135:105357  WB ;  Human.  
[IF=6.832] Hou, Yonghui. et al. Nonwoven-based gelatin/polycaprolactone membrane loaded with ERK inhibitor U0126 for treatment of tendon defects. Stem Cell Res Ther. 2022 Dec;13(1):1-11  IHC ;  Rat.  
[IF=8.724] Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039  IF,IHC ;  Mouse.  
[IF=2.639] Jiarong Yanget al. Effects of Different Doses of Calcium on the Mitochondrial Apoptotic Pathway and Rho/ROCK Signaling Pathway in the Bone of Fluorosis Rats. Biol Trace Elem Res . 2020 Jul 25.  IHC ;  rat.  
[IF=2.304] Teng X et al. Effects of Low Dietary Phosphorus on Tibia Quality and Metabolism in Caged Laying Hens. Prev Vet Med . 2020 Aug;181:105049.  WB ;  Hen.  
[IF=0] Hub Y et al. Biomimetic fabrication of Icariin loaded nano hydroxyapatite reinforced bioactive porous scaffolds for bone regeneration. Chemical Engineering Journal, 394, 124895.  IHC ;  rat.  
[IF=8.097] Hu Q et al. Demineralized Bone Scaffolds with Tunable Matrix Stiffness for Efficient Bone Integration. ACS Appl Mater Interfaces. 2018 Aug 22;10(33):27669-27680.   IHC-P ;  Rabbit.  
[IF=3.234] Fan S et al. Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway.Life Sci. 2018 Aug 15;207:205-211.  WB ;  Rat.  
[IF=3.08] Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017).  WB ;  Human.  
[IF=3.768] Ohsugi Y et al. Evaluation of bone healing following Er: YAG laser ablation in rat calvaria compared with bur drilling.(2018)J Biophotonics.Oct 15:e201800245.  IHC ;  Rat.  
[IF=5.923] Sachin Chaugule. et al. Deubiquitinating Enzyme USP8 Is Essential for Skeletogenesis by Regulating Wnt Signaling. Int J Mol Sci. 2021 Jan;22(19):10289  IF ;  mouse.  
[IF=7.242] Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 Mar;:  IF ;  Mouse.  
[IF=2.985] Jiating Lin. et al. Metformin promotes the osseointegration of titanium implants under osteoporotic conditions by regulating BMSCs autophagy, and osteogenic differentiation. Biochem Bioph Res Co. 2020 Oct;531:228  WB ;  Rat.  
[IF=1.931] Yongtao Li. et al. Effects of epigallocatechin gallate (EGCG) on the biological properties of human dental pulp stem cells and inflammatory pulp tissue. Arch Oral Biol. 2021 Mar;123:105034  WB ;  Human.  
[IF=1.476] Kanata IBI. et al. Spontaneous extraskeletal osteosarcoma in the neck skeletal muscle of a Crlj:CD1 (ICR) mouse. 2021 Jan 18  IHC ;  Rat, Mouse.  
[IF=3.496] Liu Y et al. Fabrication of Biomolecule-Loaded Composite Scaffolds Carried by Extracellular Matrix Hydrogel  IF ;  Human.  
[IF=3.485] Bai J et al. Irradiation-induced senescence of bone marrow mesenchymal stem cells aggravates osteogenic differentiation dysfunction via paracrine signaling. Am J Physiol Cell Physiol . 2020 May 1;318(5):C1005-C1017.  FCM ;  rat.  
[IF=3.331] Ning T et al. Aging affects the proliferation and mineralization of rat dental pulp stem cells under inflammatory conditions. Int Endod J. 2019 Aug 16.  WB&IHC-P ;  Rat.  
[IF=3.373] Quade M et al. Strontium enhances BMP-2 mediated bone regeneration in a femoral murine bone defect model. J Biomed Mater Res B Appl Biomater. 2019 Apr 5.  ICC ;  Mouse.  
研究领域 免疫学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse,  (predicted: Rat, Dog, Cow, Horse, )
产品应用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 11kDa
细胞定位 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human BGP: 21-80/100 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite.

Function:
Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

Subcellular Location:
Secreted.

Post-translational modifications:
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Similarity:
Belongs to the osteocalcin/matrix Gla protein family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.

SWISS:
P02818

Gene ID:
632

Database links:

Entrez Gene: 281646 Cow

Entrez Gene: 632 Human

Entrez Gene: 397530 Pig

Entrez Gene: 25295 Rat

Omim: 112260 Human

SwissProt: P02820 Cow

SwissProt: P02818 Human

SwissProt: Q8HYY9 Pig

SwissProt: P04640 Rat

Unigene: 654541 Human

Unigene: 9722 Rat




骨钙素又称骨γ-羧谷氨酸包含蛋白
产品图片
Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse osteosarcoma labeled with Anti-Osteocalcin Polyclonal Antibody, Unconjugated (bs-0470R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Tissue/cell: bone of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Osteocalcin Polyclonal Antibody, Unconjugated(bs-0470R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-Osteocalcin antibody (bs-0470R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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